acrylamide gel

简明释义

丙烯酰胺凝胶

英英释义

例句

1.The quality of the acrylamide gel can greatly affect the resolution of the results.

丙烯酰胺凝胶的质量会极大影响结果的分辨率。

2.The researcher prepared a fresh batch of acrylamide gel for the upcoming experiment.

研究人员为即将进行的实验准备了一批新鲜的丙烯酰胺凝胶。

3.In the lab, we used acrylamide gel to separate proteins by size during electrophoresis.

在实验室，我们使用丙烯酰胺凝胶通过电泳按大小分离蛋白质。

4.To visualize DNA fragments, we stained the acrylamide gel with ethidium bromide.

为了可视化DNA片段，我们用溴化乙锭染色了丙烯酰胺凝胶。

5.After running the samples, we carefully removed the acrylamide gel from the electrophoresis apparatus.

在运行样品后，我们小心地从电泳设备中取出丙烯酰胺凝胶。

作文

Acrylamide gel is a crucial component in the field of biochemistry and molecular biology. It serves as a medium for separating proteins and nucleic acids through a process known as gel electrophoresis. This technique is vital for researchers who aim to analyze the composition of biological samples. The use of acrylamide gel (丙烯酰胺凝胶) allows for high-resolution separation of macromolecules, which is essential for various applications, including protein purification, DNA analysis, and the study of genetic variations.The preparation of acrylamide gel (丙烯酰胺凝胶) involves mixing acrylamide with a cross-linking agent, typically bis-acrylamide, along with a buffer solution. Once polymerized, this mixture forms a gel-like substance that can be cast into molds to create wells for sample loading. The concentration of acrylamide used in the gel affects the pore size, which in turn influences the separation of different sized molecules. For instance, lower concentrations are suitable for separating larger proteins, while higher concentrations are better for smaller peptides and nucleic acids.One of the significant advantages of using acrylamide gel (丙烯酰胺凝胶) is its versatility. Researchers can adjust the gel's properties by altering the acrylamide concentration, allowing for customized conditions tailored to specific experiments. This flexibility makes it an indispensable tool in laboratories worldwide. Moreover, the clarity of the gel enables easy visualization of bands after electrophoresis, often enhanced by staining techniques such as Coomassie Brilliant Blue or silver staining.In addition to its role in protein and DNA analysis, acrylamide gel (丙烯酰胺凝胶) is also utilized in Western blotting, a method used to detect specific proteins in a sample. After separating proteins via gel electrophoresis, they are transferred onto a membrane where they can be probed with antibodies. This technique has revolutionized the study of protein expression and function, making acrylamide gel (丙烯酰胺凝胶) a fundamental part of modern biological research.However, it is essential to handle acrylamide gel (丙烯酰胺凝胶) with care due to the potential health risks associated with acrylamide, which is a neurotoxin and a possible carcinogen. Proper laboratory safety protocols must be followed when preparing and disposing of acrylamide gels to minimize exposure and environmental impact. Researchers must wear gloves, masks, and protective eyewear when working with acrylamide solutions.In conclusion, acrylamide gel (丙烯酰胺凝胶) is an essential tool in the scientific community, facilitating the separation and analysis of biomolecules. Its ability to provide high-resolution results makes it invaluable for various applications in research and diagnostics. As science continues to advance, the role of acrylamide gel (丙烯酰胺凝胶) will undoubtedly remain significant, contributing to our understanding of complex biological systems and the development of new therapeutic strategies.

丙烯酰胺凝胶是生物化学和分子生物学领域的重要组成部分。它作为一种介质，通过称为凝胶电泳的过程分离蛋白质和核酸。这项技术对于希望分析生物样本组成的研究人员至关重要。使用丙烯酰胺凝胶（acrylamide gel）可以实现大分子的高分辨率分离，这对包括蛋白质纯化、DNA分析和遗传变异研究在内的各种应用都是必不可少的。丙烯酰胺凝胶（acrylamide gel）的制备涉及将丙烯酰胺与交联剂（通常是双丙烯酰胺）以及缓冲溶液混合。在聚合后，这种混合物形成一种类似凝胶的物质，可以铸造成模具，以创建用于样品加载的孔。凝胶中使用的丙烯酰胺浓度会影响孔径，从而影响不同大小分子的分离。例如，较低浓度适合分离较大的蛋白质，而较高浓度则更适合较小的肽和核酸。使用丙烯酰胺凝胶（acrylamide gel）的一个显著优点是其多功能性。研究人员可以通过改变凝胶的属性来调整其性能，从而为特定实验量身定制条件。这种灵活性使其成为全球实验室中不可或缺的工具。此外，凝胶的清晰度使得在电泳后容易可视化条带，通常通过考马斯亮蓝或银染等染色技术增强。除了在蛋白质和DNA分析中的作用外，丙烯酰胺凝胶（acrylamide gel）还用于西方印迹法，这是一种用于检测样本中特定蛋白质的方法。在通过凝胶电泳分离蛋白质后，它们被转移到膜上，在那里可以用抗体探测。这项技术彻底改变了对蛋白质表达和功能的研究，使得丙烯酰胺凝胶（acrylamide gel）成为现代生物研究中的基础部分。然而，处理丙烯酰胺凝胶（acrylamide gel）时必须小心，因为丙烯酰胺可能具有神经毒性和致癌性。因此，在准备和处置丙烯酰胺凝胶时，必须遵循适当的实验室安全协议，以尽量减少暴露和环境影响。研究人员在处理丙烯酰胺溶液时必须佩戴手套、口罩和防护眼镜。总之，丙烯酰胺凝胶（acrylamide gel）是科学界的重要工具，促进了生物大分子的分离和分析。其提供高分辨率结果的能力使其在研究和诊断的各种应用中都具有不可估量的价值。随着科学的不断进步，丙烯酰胺凝胶（acrylamide gel）的作用无疑将继续显著，助力我们理解复杂的生物系统并开发新的治疗策略。