terminal transferase

简明释义

末端转移酶

英英释义

例句

1.Researchers often utilize terminal transferase for labeling DNA fragments in various experiments.

研究人员经常使用末端转移酶为不同实验中的DNA片段进行标记。

2.In molecular biology, terminal transferase is used to add nucleotides to the 3' end of a DNA strand.

在分子生物学中，末端转移酶用于向DNA链的3'末端添加核苷酸。

3.The activity of terminal transferase can be influenced by the presence of different metal ions.

末端转移酶的活性可能会受到不同金属离子存在的影响。

4.The terminal transferase enzyme can be crucial for synthesizing polyadenylated RNA.

末端转移酶酶对于合成多腺苷酸化RNA至关重要。

5.Using terminal transferase, scientists can create custom oligonucleotides for therapeutic applications.

科学家可以使用末端转移酶为治疗应用创建定制的寡核苷酸。

作文

Terminal deoxynucleotidyl transferase, often abbreviated as terminal transferase, is an enzyme that plays a crucial role in molecular biology and genetic research. This enzyme is particularly known for its ability to add nucleotides to the 3' ends of a DNA molecule without a template. This unique property makes terminal transferase an invaluable tool in various applications, including DNA sequencing, labeling, and the synthesis of DNA probes.The mechanism of action of terminal transferase involves the incorporation of deoxynucleotides into a growing DNA strand. Unlike other polymerases that require a template strand to guide the synthesis, terminal transferase can function independently, which allows researchers to create long stretches of homopolymeric tails on the ends of DNA fragments. This feature is particularly useful when preparing samples for cloning or when generating specific DNA constructs.In the laboratory, terminal transferase is used extensively in the preparation of DNA for various experiments. For instance, it can be employed to add a poly-A tail to mRNA molecules, enhancing their stability and facilitating their subsequent analysis. Additionally, terminal transferase is often utilized in the labeling of DNA probes with fluorescent tags, which are essential for detecting specific sequences in hybridization assays.The versatility of terminal transferase extends beyond basic research; it has significant applications in clinical diagnostics and therapeutic developments. For example, it can be used to create DNA constructs that are essential for gene therapy, where modified genes are introduced into a patient's cells to treat genetic disorders. The ability of terminal transferase to generate specific DNA sequences also aids in the development of personalized medicine, where treatments are tailored to individual genetic profiles.Moreover, the study of terminal transferase has led to advancements in understanding enzyme kinetics and regulation. Researchers have investigated the factors that influence its activity, including temperature, pH, and substrate concentration. This knowledge not only enhances the efficiency of laboratory techniques but also contributes to our overall understanding of enzymatic processes in biological systems.In conclusion, terminal transferase is a pivotal enzyme in the field of molecular biology. Its ability to add nucleotides to the ends of DNA without a template opens up a myriad of possibilities for scientific research and clinical applications. As technology advances, the use of terminal transferase is likely to expand further, paving the way for innovative solutions in genetics and biotechnology. Understanding the functions and applications of this enzyme is essential for anyone involved in molecular biology, as it underscores the intricate relationship between enzymes and genetic material, ultimately contributing to the progress of science and medicine.

终端脱氧核苷酸转移酶，通常缩写为terminal transferase，是一种在分子生物学和基因研究中发挥重要作用的酶。这种酶以其能够在没有模板的情况下向DNA分子的3'末端添加核苷酸而闻名。这一独特的特性使得terminal transferase成为各种应用中不可或缺的工具，包括DNA测序、标记以及DNA探针的合成。terminal transferase的作用机制涉及将脱氧核苷酸纳入生长的DNA链中。与其他需要模板链来指导合成的聚合酶不同，terminal transferase可以独立运行，这使得研究人员能够在DNA片段的末端创建长的同聚体尾。这一特征在为克隆样本准备或生成特定DNA构建时尤其有用。在实验室中，terminal transferase广泛用于准备DNA以进行各种实验。例如，它可以用于向mRNA分子添加聚A尾，从而增强其稳定性并促进后续分析。此外，terminal transferase常用于将荧光标签标记到DNA探针上，这对于在杂交检测中特定序列的检测至关重要。terminal transferase的多功能性超越了基础研究；它在临床诊断和治疗开发中也具有重要应用。例如，它可以用于创建对基因治疗至关重要的DNA构建，其中修饰的基因被引入患者细胞中以治疗遗传疾病。terminal transferase生成特定DNA序列的能力也有助于个性化医学的发展，即根据个体的基因特征量身定制治疗方案。此外，对terminal transferase的研究推动了对酶动力学和调控的理解。研究人员调查了影响其活性的因素，包括温度、pH和底物浓度。这些知识不仅提高了实验室技术的效率，还促进了我们对生物系统中酶过程的整体理解。总之，terminal transferase是分子生物学领域的关键酶。它在没有模板的情况下向DNA末端添加核苷酸的能力为科学研究和临床应用开辟了无数可能性。随着技术的进步，terminal transferase的使用可能会进一步扩展，为基因学和生物技术的创新解决方案铺平道路。理解这种酶的功能和应用对于任何参与分子生物学的人来说都是必不可少的，因为它强调了酶与遗传物质之间的复杂关系，最终促进了科学和医学的进步。