supernatant liquid

简明释义

上层清液

英英释义

The liquid that remains above the solid residue after centrifugation or sedimentation.

离心或沉淀后,固体残留物上方剩余的液体。

例句

1.During the filtration process, the supernatant liquid 上清液 was collected in a separate container.

在过滤过程中,supernatant liquid上清液被收集到一个单独的容器中。

2.We need to measure the pH of the supernatant liquid 上清液 before proceeding.

在继续之前,我们需要测量supernatant liquid上清液的pH值。

3.The analysis of the supernatant liquid 上清液 revealed high levels of contaminants.

supernatant liquid上清液的分析显示出高水平的污染物。

4.After centrifugation, the supernatant liquid 上清液 was carefully removed from the sediment.

离心后,小心地将supernatant liquid上清液从沉淀物中取出。

5.The supernatant liquid 上清液 contains the soluble proteins needed for the experiment.

supernatant liquid上清液包含实验所需的可溶性蛋白质。

作文

In the field of chemistry and biology, the term supernatant liquid refers to the clear liquid that remains above the solid residue after a mixture has been allowed to settle or has been centrifuged. Understanding this concept is crucial for various laboratory procedures, including the separation of different components in a solution. For example, when cells are lysed in a laboratory experiment, the cellular debris settles at the bottom of the tube, while the supernatant liquid contains soluble proteins, nucleic acids, and other biomolecules that can be further analyzed. This separation technique is not only fundamental in research but also plays a significant role in medical diagnostics, where the analysis of supernatant liquid can provide insights into a patient’s health status.The process of obtaining a supernatant liquid typically involves either gravity settling or centrifugation. In gravity settling, the mixture is left undisturbed for a period, allowing denser particles to fall to the bottom. In contrast, centrifugation accelerates this process by spinning the mixture at high speeds, which generates a force that separates the components based on their densities. The liquid that remains on top after this process is what we refer to as supernatant liquid.One common application of supernatant liquid is in the preparation of cell cultures. When culturing cells, it is essential to remove dead cells and debris to ensure that the culture remains healthy. After allowing the culture to settle, researchers can carefully pipette the supernatant liquid to transfer only the viable cells to a new container. This practice helps maintain the integrity of the culture and promotes optimal growth conditions.Additionally, the analysis of supernatant liquid is vital in biochemical assays. For instance, in enzyme activity assays, the reaction mixture may contain substrates, enzymes, and other reagents. After the reaction has taken place, the supernatant liquid is collected to measure the concentration of products formed. This information is essential for understanding enzyme kinetics and the effectiveness of inhibitors or activators.Moreover, the supernatant liquid can also be utilized in environmental studies. For example, when analyzing soil or sediment samples, researchers often extract the supernatant liquid to assess the presence of pollutants or nutrients. By studying the composition of this liquid, scientists can gain insights into the ecological health of an environment and make informed decisions regarding conservation efforts.In summary, the term supernatant liquid is integral to many scientific disciplines, particularly in laboratory settings. It represents the liquid phase that remains after a solid phase has settled, allowing for the isolation and analysis of various substances. Whether in cell culture, biochemical assays, or environmental studies, understanding how to effectively work with supernatant liquid is essential for obtaining accurate and meaningful results. As science continues to advance, the importance of mastering such concepts will only grow, highlighting the need for education and training in these fundamental techniques.

在化学和生物学领域,术语supernatant liquid指的是在混合物被允许沉淀或经过离心处理后,固体残留物上方的清液。理解这个概念对于各种实验室程序至关重要,包括分离溶液中的不同成分。例如,当细胞在实验室实验中被裂解时,细胞碎片会沉淀在试管底部,而supernatant liquid则包含可溶性蛋白质、核酸和其他生物分子,这些都可以进一步分析。这种分离技术不仅在研究中是基础,同时在医学诊断中也发挥着重要作用,分析supernatant liquid可以提供患者健康状况的见解。获得supernatant liquid的过程通常涉及重力沉降或离心。在重力沉降中,混合物在一段时间内静置,让更密集的颗粒沉到底部。相反,离心通过以高速旋转混合物来加速这一过程,这产生了一种基于密度分离组分的力。这个过程后,上面剩下的液体就是我们所称的supernatant liquidsupernatant liquid的一个常见应用是在细胞培养的准备过程中。当培养细胞时,去除死细胞和碎屑对于确保培养健康至关重要。在让培养物沉淀后,研究人员可以小心地用移液器吸取supernatant liquid,将仅存活的细胞转移到新的容器中。这一做法有助于保持培养的完整性,并促进最佳生长条件。此外,分析supernatant liquid在生化分析中也是至关重要的。例如,在酶活性分析中,反应混合物可能包含底物、酶和其他试剂。反应完成后,收集supernatant liquid以测量生成的产物浓度。这些信息对于理解酶动力学及抑制剂或激活剂的有效性至关重要。此外,supernatant liquid在环境研究中也可以被利用。例如,在分析土壤或沉积物样品时,研究人员常常提取supernatant liquid以评估污染物或养分的存在。通过研究这种液体的成分,科学家可以深入了解环境的生态健康,并就保护工作做出明智的决定。总之,术语supernatant liquid在许多科学学科中都是不可或缺的,特别是在实验室环境中。它代表了在固体相沉淀后剩余的液相,从而使各种物质的分离和分析成为可能。无论是在细胞培养、生化分析还是环境研究中,理解如何有效地处理supernatant liquid对于获得准确和有意义的结果至关重要。随着科学的不断进步,掌握这些概念的重要性只会增加,这突显了在这些基础技术方面进行教育和培训的必要性。

相关单词

supernatant

supernatant详解:怎么读、什么意思、用法