reversed phase

简明释义

反相

英英释义

例句

1.The reversed phase 反相 technique allows for the separation of polar and non-polar molecules effectively.

该反相 reversed phase 技术能有效分离极性和非极性分子。

2.In pharmaceutical analysis, reversed phase 反相 chromatography is widely used for drug purity testing.

在药物分析中，反相 reversed phase 色谱法广泛用于药物纯度测试。

3.The reversed phase 反相 method is preferred for analyzing non-polar compounds in complex mixtures.

在复杂混合物中分析非极性化合物时，优先采用反相 reversed phase 方法。

4.By using a reversed phase 反相 column, we can achieve better resolution of the analytes.

通过使用反相 reversed phase 柱，我们可以实现更好的分析物分辨率。

5.In liquid chromatography, we often use reversed phase 反相 techniques to separate compounds based on their hydrophobicity.

在液相色谱中，我们常用反相 reversed phase 技术根据化合物的疏水性进行分离。

作文

In the field of analytical chemistry, the term reversed phase refers to a specific type of chromatography technique that is widely used for separating non-volatile compounds. This method contrasts with normal phase chromatography, where the stationary phase is polar and the mobile phase is non-polar. In reversed phase chromatography, however, the stationary phase is non-polar, while the mobile phase is polar. This fundamental difference allows for the effective separation of a wide range of substances, particularly in liquid chromatography applications.The principle behind reversed phase chromatography revolves around the interactions between the analytes (the substances being measured) and the stationary and mobile phases. Since the stationary phase is non-polar, it tends to interact more strongly with non-polar compounds, allowing these compounds to be retained longer on the column. Conversely, polar compounds will have a shorter retention time because they interact more favorably with the polar mobile phase. This selective retention based on polarity makes reversed phase chromatography an invaluable tool in many laboratories.One of the most significant advantages of using reversed phase chromatography is its versatility. It can be employed to analyze a variety of samples, including pharmaceuticals, environmental samples, and biological fluids. For example, in pharmaceutical analysis, scientists often need to separate and quantify active ingredients in drug formulations. The reversed phase technique allows for precise separation, enabling accurate analysis and quality control.Moreover, the choice of solvents in reversed phase chromatography plays a crucial role in the effectiveness of the separation process. Commonly used solvents include water mixed with organic solvents such as acetonitrile or methanol. By adjusting the composition of the mobile phase, chemists can optimize the separation of complex mixtures. This adaptability is particularly beneficial when dealing with samples that contain a wide range of polarities.Another important aspect of reversed phase chromatography is the use of various stationary phase materials. Different types of bonded phases, such as C18, C8, or phenyl, can be used depending on the specific requirements of the analysis. Each type provides distinct selectivity and retention characteristics, allowing chemists to tailor their approach based on the nature of the compounds being analyzed.In conclusion, reversed phase chromatography serves as a cornerstone technique in analytical chemistry, providing researchers with a powerful means of separating and analyzing complex mixtures. Its ability to accommodate a wide variety of sample types, coupled with the flexibility in solvent selection and stationary phase materials, makes it an essential tool in both academic research and industrial applications. As the demand for precise and efficient analytical methods continues to grow, the importance of reversed phase chromatography will undoubtedly remain significant in the future of chemical analysis.

在分析化学领域，术语reversed phase指的是一种广泛用于分离非挥发性化合物的特定色谱技术。这种方法与正常相色谱形成对比，后者的固定相是极性的，而流动相是非极性的。然而，在reversed phase色谱中，固定相是非极性的，而流动相是极性的。这一根本差异使得有效分离各种物质成为可能，特别是在液相色谱应用中。reversed phase色谱背后的原理围绕着分析物（被测量的物质）与固定相和流动相之间的相互作用展开。由于固定相是非极性的，它往往与非极性化合物之间的相互作用更强，从而使这些化合物在柱上保留的时间更长。相反，极性化合物的保留时间较短，因为它们与极性流动相的相互作用更为有利。这种基于极性的选择性保留使得reversed phase色谱成为许多实验室中不可或缺的工具。使用reversed phase色谱的一个显著优势是其多功能性。它可以用于分析多种样品，包括药品、环境样品和生物体液。例如，在药物分析中，科学家通常需要分离和定量药物制剂中的活性成分。reversed phase技术允许精确分离，从而实现准确的分析和质量控制。此外，reversed phase色谱中溶剂的选择在分离过程的有效性中起着至关重要的作用。常用的溶剂包括水与有机溶剂（如乙腈或甲醇）的混合物。通过调整流动相的组成，化学家可以优化复杂混合物的分离。这种适应性在处理包含广泛极性的样品时尤其有益。reversed phase色谱的另一个重要方面是各种固定相材料的使用。根据分析的具体要求，可以使用不同类型的键合相，如C18、C8或苯基。每种类型提供独特的选择性和保留特性，使化学家能够根据所分析化合物的性质量身定制他们的方法。总之，reversed phase色谱作为分析化学的基石技术，为研究人员提供了一种强大的手段，用于分离和分析复杂混合物。其能够容纳各种样品类型，加上溶剂选择和固定相材料的灵活性，使其成为学术研究和工业应用中的基本工具。随着对精确和高效分析方法的需求不断增长，reversed phase色谱的重要性无疑将在化学分析的未来中保持显著。