counterstain

简明释义

英[ˈkaʊntəˌsteɪn]美[ˈkaʊntərˌsteɪn]

n. [生物] 复染色（指作显微镜观察时使用的一种通过第二次染色使生物标本显示不同颜色的染色剂）

英英释义

单词用法

同义词

反义词

例句

1.Labelling microcircumstance, the size of vessel opening diameter and counterstain may also affect the result.

标记微环境，血管开口大小和复染可能影响标记结果。

2.Labelling microcircumstance, the size of vessel opening diameter and counterstain may also affect the result.

标记微环境，血管开口大小和复染可能影响标记结果。

3.A common counterstain 对照染色 in microbiology is safranin, which stains gram-negative bacteria.

微生物学中常见的counterstain 对照染色 是红色素，它给革兰氏阴性细菌染色。

4.After applying the primary stain, you should use a counterstain 对照染色 to highlight the structures of interest.

在应用主要染色后，你应该使用一个counterstain 对照染色 来突出感兴趣的结构。

5.Using a different color for the counterstain 对照染色 can help distinguish between various cell types.

使用不同颜色的counterstain 对照染色 可以帮助区分不同的细胞类型。

6.The technician decided to apply a counterstain 对照染色 to visualize the cell nuclei better.

技术员决定应用一个counterstain 对照染色 来更好地可视化细胞核。

7.In histology, a counterstain 对照染色 is often used to provide contrast to the primary stain.

在组织学中，counterstain 对照染色 通常用于为主要染色提供对比。

作文

In the field of biology, especially in microbiology, the process of staining is crucial for visualizing cells and their components under a microscope. One of the essential techniques used in this context is known as counterstain, which plays a significant role in enhancing the visibility of certain structures within the sample. A counterstain (对比染色) is typically applied after the primary stain to provide contrast and help distinguish different cell types or structures. This method is essential when dealing with complex samples that contain various cellular components, as it allows researchers to observe specific features more clearly.The application of a counterstain can be seen in various staining protocols, such as the Gram stain, which is used to classify bacteria into two groups: Gram-positive and Gram-negative. In this procedure, the primary stain (crystal violet) is first applied, followed by a decolorization step. After decolorization, a counterstain (对比染色) like safranin is added to provide contrast. The Gram-positive bacteria retain the crystal violet stain and appear purple, while the Gram-negative bacteria take up the counterstain and appear pink. This differentiation is vital for identifying bacterial species and determining appropriate treatment methods.Moreover, the use of counterstain (对比染色) is not limited to bacterial studies. In histology, where tissue samples are examined, a counterstain can help highlight particular cell types or structures within the tissue. For instance, in immunohistochemistry, a primary antibody may label specific proteins in the sample. Following this, a counterstain (对比染色) such as hematoxylin can be used to stain the nuclei of all cells, providing a background that enhances the visibility of the labeled proteins. This combination allows researchers to visualize the distribution and expression levels of proteins in relation to the overall tissue architecture.The importance of counterstain (对比染色) extends beyond mere visualization; it also aids in the interpretation of results. By providing clear contrasts between different components, it allows scientists to make more accurate assessments of cellular morphology and function. Without the application of a counterstain, many microscopic observations would be challenging, as various structures might blend together, leading to misinterpretations.In conclusion, the use of counterstain (对比染色) is a fundamental aspect of biological staining techniques, significantly enhancing the clarity and accuracy of microscopic observations. Whether in microbiology or histology, counterstain provides the necessary contrast to distinguish between different structures, making it an invaluable tool for researchers. As we continue to explore the complexities of life at the microscopic level, the role of counterstain (对比染色) will undoubtedly remain critical in advancing our understanding of biology.

在生物学领域，尤其是微生物学中，染色过程对于在显微镜下观察细胞及其成分至关重要。在这种情况下，使用的基本技术之一被称为counterstain（对比染色），它在增强样本中特定结构的可视性方面发挥着重要作用。counterstain（对比染色）通常在主要染色后应用，以提供对比并帮助区分不同的细胞类型或结构。当处理包含各种细胞成分的复杂样本时，这种方法至关重要，因为它使研究人员能够更清晰地观察特定特征。counterstain（对比染色）的应用可以在各种染色协议中看到，例如革兰氏染色，该染色用于将细菌分为两组：革兰阳性和革兰阴性。在此过程中，首先施加主要染料（结晶紫），然后进行脱色步骤。脱色后，添加counterstain（对比染色），如沙黄染料，以提供对比。革兰阳性细菌保留结晶紫染料，呈紫色，而革兰阴性细菌则吸收counterstain（对比染色），呈粉红色。这种区分对于识别细菌种类和确定适当的治疗方法至关重要。此外，counterstain（对比染色）的使用不限于细菌研究。在组织学中，检查组织样本时，counterstain（对比染色）可以帮助突出组织中的特定细胞类型或结构。例如，在免疫组化中，初级抗体可能标记样本中的特定蛋白质。随后，可以使用counterstain（对比染色），如苏木精，对所有细胞的细胞核进行染色，提供增强标记蛋白可视性的背景。这种组合使研究人员能够可视化蛋白质的分布和表达水平与整体组织结构之间的关系。counterstain（对比染色）的重要性不仅限于单纯的可视化；它还有助于结果的解释。通过提供不同成分之间的清晰对比，它使科学家能够更准确地评估细胞形态和功能。如果不应用counterstain（对比染色），许多显微观察将会变得具有挑战性，因为各种结构可能会混合在一起，导致误解。总之，counterstain（对比染色）的使用是生物染色技术的基本方面，显著增强了显微观察的清晰度和准确性。无论是在微生物学还是组织学中，counterstain提供了必要的对比，以区分不同的结构，使其成为研究人员不可或缺的工具。随着我们继续探索微观层面生命的复杂性，counterstain（对比染色）的角色无疑将在推动我们对生物学的理解中保持关键。